Letteratura scientifica

Abstract

 

Objectives: Platelet-rich fibrin (PRF)-based membranes have been used for covering alveolar ridge augmentation side in several in vivo studies. Few in vitro studies on PRF and no studies using human periosteal cells for tissue engineering have been published. The aim is a comparison of PRF with the commonly used collagen membrane Bio-Gides as scaffolds for periosteal tissue engineering.

 

Material and methods: Human periosteal cells were seeded on membrane pieces (collagen [Bio-Gides] and PRF) at a density of 104 cells/well. Cell vitality was assessed by fluorescein diacetate (FDA) and propidium iodide (PI) staining, biocompatibility with the lactate dehydrogenase (LDH) test and proliferation level with the MTT, WST and BrdU tests and scanning electron microscopy (SEM).

 

Results: PRF membranes showed slightly inferior biocompatibility, as shown by the LDH test. The metabolic activity measured by the MTT and WST tests was higher for PRF than for collagen (BioGides). The proliferation level as measured by the BrdU test (quantitative) and SEM examinations (qualitative) revealed higher values for PRF.

 

Conclusion: PRF appears to be superior to collagen (Bio-Gides) as a scaffold for human periosteal cell proliferation. PRF membranes are suitable for in vitro cultivation of periosteal cells for bone tissue engineering.

Platelet-rich fibrin membranes as scaffolds for periosteal tissue engineering

 

 

Date: Accepted 4 November 2009. Clin. Oral Impl. Res. 21, 2010; 543–549. doi: 10.1111/j.1600-0501.2009.01900.x

Key words: collagen membrane, periosteum, platelet-rich fibrin membrane, stem cell, tissue engineering

Regenerative

Medicine: 8

SUPPORTO
SERVIZIO CLIENTI
L-PRF™: INFORMAZIONI PER IL PAZIENTE
PRODOTTI: ISTRUZIONI D'USO
DRIVE-LOCK
CONFEZIONAMENTO
GUIDA DI TORQUE
VIDEO
ALTRI PRODOTTI
REOSS
BIOPAD
EXOMED
IMPLANT REMOVER
TORQUE LOCK
BONE PROFILER
KIT ABUTMENT TRY-IN
SOLUZIONI DIGITALI
NETWORK
DISTRIBUTORI
CORSI ED EVENTI
SEGUICI SU FACEBOOK 
SEGUICI SU YOUTUBE 
SEGUICI SU LINKEDIN 
AZIENDA
BIOMIMETIC
NEWS
CERTIFICAZIONI

Servizio Vendita: +39 089-233045

Lunedì - Venerdì, 9:00 a.m. - 6:00 p.m. |  ITALIA

Via Fabrizio Pinto, 16 * 84124 Salerno (SA) Italy

Area Riservata

©2016 Intra-Lock International       |   Made in the USA